Le Ana細胞
生長狀態(tài)： 貼壁生長
ATCC Number： CRL-1192?
相關**： 馬凡氏綜合征
數(shù)量： 大量
器官來源： 皮膚
年限： 2 years
運輸方式： 凍存運輸
細胞形態(tài)： 成纖維樣
是否是腫瘤細胞： 0
Le Ana細胞物種來源： 人
Designations: Le Ana
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Le Ana細胞Marfan syndrome
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 2 years
Gender: female
Comments: The tissue donor suffered from an atypical form of Marfan syndrome.
The patient presented with no eye findings, normal height and severe joint laxity.
Propagation: Le Ana細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Le Ana細胞Subculture every 7 to 9 days.
