ATCC Number： HB-12317?
相關(guān)**： 其他**
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
細(xì)胞形態(tài)： 其他
數(shù)量： 大量
器官來源： 大腦
NG108-15細(xì)胞Designations: NG108-15 [108CC15]
Depositors: Univ. Texas Southwestern Medical Cntr.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (neuroblastoma); Rattus norvegicus (glioma) deposited as mouse (neuroblastoma); rat (glioma)
Morphology: flat; round; 10 to 100 micrometers diameter
Source: Organ: brain
Disease: glioblastoma; neuroblastoma
Cell Type: somatic cell hybrid
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host NG108-15細(xì)胞(Roche Transfection Reagents)
Comments: The NG108-15 cell line, originally named 108CC15, was developed in 1971 by Bernd Hamprecht. [112482]
The line was formed by fusing mouse N18TG2 neuroblastoma cells with rat C6-BU-1 glioma cells in the presence of inactivated Sendai virus. [51493]
Propagation: ATCC complete growth medium: The base medium for this cell line is Dulbecco's Modified Eagle's Medium (GIBCO/InVitrogen Catalog No.12100-061, DMEM without sodium pyruvate ). To make the complete growth medium, add the following components to the base medium:
0.1 mM hypoxanthine (final conc.)
400 nM aminopterin (final conc.)
0.016 mM thymidine (final conc.)
10% fetal bovine serum (final conc.)
1.5 g/L sodium bicarbonate
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. NG108-15細(xì)胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
References: 51492: Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829
51493: Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. NG108-15細(xì)胞PubMed: 2985920
112482: Bernd Hamprecht, personal communication
