147-67C6細(xì)胞

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG1 and IgG2b

ATCC Number： CRL-2654?

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

Designations: 147-67C6

147-67C6細(xì)胞Depositors: National Cancer Institute

Isotype: IgG1 and IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against a synthetic v-ras K oncogene peptide

Permits/Forms: 147-67C6細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with a synthetic peptide with the sequence YTLVREIRQYRLKKESKEEKTPGC corresponding to peptides 157-180 of the v-ras K oncogene.

Spleen cells were fused with Sp2/0 myeloma cells.

The antibody reacts with the synthetic v-ras K oncogene peptide. In ELISA testing, the antibody weakly detects the homologous region in the ras H sequence. In immunoblot testing a serum protein of approximately 45 kd is detected.

The IgG1 and IgG2b reactivities of the antibody with the v-ras K peptide (157-180) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. 147-67C6細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium 147-67C6細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020