TGP55細(xì)胞
數(shù)量： 大量
細(xì)胞形態(tài)： 上皮樣
年限： 7.5 months
運輸方式： 凍存運輸
是否是腫瘤細(xì)胞： 0
物種來源： 轉(zhuǎn)基因小鼠
生長狀態(tài)： 貼壁生長
器官來源： 胰腺
ATCC Number： CRL-2150?
TGP55細(xì)胞相關(guān)**： 其他**
品系： Tg(E1a-1-SV40E)Bri18
Designations: TGP55
Depositors: OS Pettengill, D Longnecker
Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus, transgenic deposited as mouse, transgenic
Morphology: epithelial
Source: Organ:TGP55細(xì)胞 pancreas
Strain: Tg(E1a-1-SV40E)Bri18
Disease: pancreatic small cell tumor
Cellular Products: insulin; somatostatin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 7.5 months
Gender: female
Comments: TGP55 is an epithelial-like cell line derived from a pancreatic tumor with small cell anaplastic morphology that arose in an ***** female transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the coding region for the SV40 T antigen.
TGP55細(xì)胞The cells do not become confluent.
They grow very slowly as islands, and should be subcultured when the islands begin to form domes.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22323: Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. TGP55細(xì)胞PubMed: 7904904
