產(chǎn)品描述
ARE熒光素酶報告基因（報告基因質(zhì)粒）（ARE luciferase reporter plasimd）是自主研發(fā)的用于檢測ARE轉(zhuǎn)錄活性水平為目的的報告基因。ARE(antioxidant response element) 與Nrf2和NRF1轉(zhuǎn)錄因子結(jié)合，調(diào)節(jié)參與保護(hù)細(xì)胞免受氧化損傷的相關(guān)基因。
ARE報告基因主要應(yīng)用于檢測細(xì)胞的抗氧化水平或氧化應(yīng)激狀態(tài)、**研究以及基因過表達(dá)和RNAi的表型分析等。
pGMARE-Lu是改造后的哺乳動物真核表達(dá)載體，在其多克隆位點(diǎn)插入了多個ARE結(jié)合位點(diǎn)，可以高靈敏度地檢測ARE的激活水平。同時，對載體中預(yù)測出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔儯黾恿速|(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。由于質(zhì)粒體積減小，使得ARE報告基因更易于轉(zhuǎn)染。ARE luciferase reporter plasmid （ARE-Luc熒光素酶報告基因質(zhì)粒）
質(zhì)粒圖譜
ARE luciferase reporter plasmid （ARE-Luc熒光素酶報告基因質(zhì)粒）運(yùn)輸與保存方法
冰袋（wet ice）運(yùn)輸。收到產(chǎn)品后放到-20 oC保存。
注意事項
本質(zhì)粒未經(jīng)允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
為了您的健康，實(shí)驗(yàn)操作時請穿實(shí)驗(yàn)服和帶一次性手套。
使用說明
pGMARE-Lu可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動物細(xì)胞。用熒光素酶檢測試劑盒或雙熒光素酶檢測試劑盒進(jìn)行檢測。
參考文獻(xiàn)ARE luciferase reporter plasmid （ARE-Luc熒光素酶報告基因質(zhì)粒）
[1] Huang Y, et al. Anti-oxidative stress regulator NF-E2-related factor 2 mediates the adaptive induction of antioxidant and detoxifying enzymes by lipid peroxidation metabolite 4-hydroxynonenal. Cell Biosci. 2012 Nov 28;2(1):40.
[2] Wright T, et al. The S349T mutation of SQSTM1 links Keap1/Nrf2 signalling to Pagets disease of bone. Bone. 2013 Feb;52(2):699-706.
[3] Yang YC, et al. Docosahexaenoic acid inhibition of inflammation is partially via cross-talk between Nrf2/hemeoxygenase 1 and IKK/NF-κB pathways.J NutrBiochem. 2013 Jan;24(1):204-12.
[4]Guseva NV, et al. P53 and the proteasome regulate androgen receptor activity. Cancer BiolTher. 2012 May 1;13(7):553-8.
[5] Tsai CW, et al. Carnosic acid induces the NAD(P)H: quinoneoxidoreductase 1 expression in rat clone 9 cells through the p38/nuclear factor erythroid-2 related factor 2 pathway. J Nutr. 2011 Dec;141(12):2119-25.
[6] Wang DJ, et al. Regulation of the let-7a-3 promoter by NF-κB. PLoS One. 7(2):e31240 (2012).
[7] Tran TA, et al. Lipopolysaccharide and tumor necrosis factor regulate Parkin expression via nuclear factor-kappa B. PLoS One. 6(8):e23660 (2011).
[8] Robbins D, et al. Imaging NF-κB signaling in mice for screening anticancer drugs. Methods Mol Biol. 716:169-77 (2011).
[9] Dvorák Z, et al. Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kappaB in HeLa cells. ChemBiol Interact. 159(2):117-28 (2006).
[10] Ozeki M, et al. Response of cyclin B1 to ionizing radiation: regulation by NF-kappaB and mitochondrial antioxidant enzyme MnSOD. Anticancer Res. 24(5A):2657-63 (2004).ARE luciferase reporter plasmid （ARE-Luc熒光素酶報告基因質(zhì)粒）
