SRE GFP Reporter Plasmid SRE-GFP報(bào)告基因質(zhì)粒
產(chǎn)品描述
SRE-GFP報(bào)告基因是自主研發(fā)的用于檢測SRF轉(zhuǎn)錄活性水平為目的的報(bào)告基因。三重復(fù)合體TCR/Elk1/SRF結(jié)合到SRE進(jìn)行基因表達(dá)的調(diào)控。
SRE-GFP報(bào)告基因主要用于檢測細(xì)胞中MAPK/ERK信號通路、**研究、基因過表達(dá)和RNAi的表型分析等。
pGMSRE-GFP是改造后的哺乳動(dòng)物真核表達(dá)載體，在其多克隆位點(diǎn)插入了多個(gè)SRE結(jié)合位點(diǎn)，可以高效地檢測SRE的激活水平。由于載體采用了GFP作為報(bào)告基因，更便于后續(xù)的檢測。同時(shí)，對載體中預(yù)測出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔?，增加了質(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外，由于質(zhì)粒體積減小，使得SRE-GFP報(bào)告基因更易于轉(zhuǎn)染。SRE GFP Reporter Plasmid SRE-GFP報(bào)告基因質(zhì)粒
質(zhì)粒圖譜
SRE GFP Reporter Plasmid SRE-GFP報(bào)告基因質(zhì)粒pGM SRE -GFP質(zhì)粒測序引物
5’-TAGCAAAATAGGCTGTCCC-3’
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃保存。
使用說明
pGMSRE-GFP可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。
注意事項(xiàng)
1）本質(zhì)粒未經(jīng)翊圣生物允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
2）為了您的健康，實(shí)驗(yàn)操作時(shí)請穿實(shí)驗(yàn)服和帶一次性手套。
SRE GFP Reporter Plasmid SRE-GFP報(bào)告基因質(zhì)粒參考文獻(xiàn)
[1] Katsch K, et al. Actin-dependent activation of serum response factor in T cells by the viral oncoprotein tip. Cell Commun Signal. 10(1):5(2012).
[2] Gozé C, et al. Involvement of tryptophan W276 and of two surrounding amino acid residues in the high constitutive activity of the ghrelin receptor GHS-R1a. Eur J Pharmacol. 643(2-3):153-61(2010).
[3] Notcovich C, et al. Histamine acting on H1 receptor promotes inhibition of proliferation via PLC, RAC, and JNK-dependent pathways. Exp Cell Res. 316(3):401-11 (2010).
[4] Hawkins JL, et al. Pharmacologic inhibition of site 1 protease activity inhibits sterol regulatory element-binding protein processing and reduces lipogenic enzyme gene expression and lipid synthesis in cultured cells and experimental animals. J Pharmacol Exp Ther. 326(3):801-8(2008).
[5] Aminova LR, et al. The C3 domain of Pasteurella multocida toxin is the minimal domain responsible for activation of Gq-dependent calcium and mitogenic signaling. Protein Sci. 17(5):945-9(2008). SRE GFP Reporter Plasmid SRE-GFP報(bào)告基因質(zhì)粒
