產(chǎn)品描述
TNF-a-promoter熒光素酶報(bào)告基因（報(bào)告基因質(zhì)粒）（TNF-a-promoter luciferase reporter plasimd）是自主研發(fā)的用于檢測(cè)human TNF-a-promoter激活水平為目的的報(bào)告基因。TNF-a（腫瘤壞死因子-α）是一種涉及到系統(tǒng)炎癥的細(xì)胞因子，主要作用是調(diào)節(jié)**細(xì)胞的功能，促使發(fā)熱，引起細(xì)胞凋亡，引發(fā)炎癥，阻止腫瘤發(fā)生和病毒復(fù)制。
TNF-a-promoter報(bào)告基因主要應(yīng)用于NF-kb信號(hào)通路的研究等和TNF-a-promoter激活水平的檢測(cè)等。
pGMTNF-a-promoter-Lu是改造后的哺乳動(dòng)物真核表達(dá)載體，在其多克隆位點(diǎn)插入了human TNF-a啟動(dòng)子，可以高靈敏度地檢測(cè)human TNF-a-promoter的激活水平。同時(shí)，對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔?，增加了質(zhì)粒對(duì)轉(zhuǎn)錄因子結(jié)合的特異性。另外，由于質(zhì)粒體積減小，使得TNF-a-promoter報(bào)告基因質(zhì)粒更易于轉(zhuǎn)染。TNF-a-promoter luciferase reporter plasmid （TNF-a-promoter-Luc熒光素酶報(bào)告基因質(zhì)粒）
質(zhì)粒圖譜
TNF-a-promoter luciferase reporter plasmid （TNF-a-promoter-Luc熒光素酶報(bào)告基因質(zhì)粒）運(yùn)輸與保存方法
冰袋（wet ice）運(yùn)輸。收到產(chǎn)品后放到-20 oC保存。
注意事項(xiàng)
本質(zhì)粒未經(jīng)允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
為了您的健康，實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。
使用說(shuō)明
pGMTNFα-promoter-Lu可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。用熒光素酶檢測(cè)試劑盒或雙熒光素酶檢測(cè)試劑盒進(jìn)行檢測(cè)。
參考文獻(xiàn)TNF-a-promoter luciferase reporter plasmid （TNF-a-promoter-Luc熒光素酶報(bào)告基因質(zhì)粒）
[1] Zhang S,et al. Epigenetic Regulation of TNFA Expression in Periodontal Disease. J Periodontol.2013 Jan 31.
[2] Simpson PD, et al. Functional differences exist between TNFα promoters encoding the common -237G SNP and the rarer HLA-B*5701-linked A variant. PLoS One. 2012;7(7):e40100.
[3] Mingala CN, et al. Comparative moleculo-immunological analysis of swamp- and riverine-type water buffaloes responses. Cytokine. 2009 May;46(2):273-82.
[4] El Gazzar MA, et al Thymoquinone attenuates proinflammatory responses in lipopolysaccharide-activated mast cells by modulating NF-kappaB nuclear transactivation BiochimBiophysActa. 2007 Apr;1770(4):556-64.
[5] Cheng N, et al. Role of transcription factor NF-kappaB in asbestos-induced TNFalpha response from macrophages. ExpMolPathol. 1999 Aug;66(3):201-10.
[6] Wang DJ, et al. Regulation of the let-7a-3 promoter by NF-κB. PLoS One. 7(2):e31240 (2012).TNF-a-promoter luciferase reporter plasmid （TNF-a-promoter-Luc熒光素酶報(bào)告基因質(zhì)粒）
