NC-37細(xì)胞

ATCC Number： CCL-214?

相關(guān)**： **瘤

細(xì)胞類型： B**細(xì)胞

細(xì)胞形態(tài)： **樣

是否是腫瘤細(xì)胞： 1

物種來源： 人

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

器官來源： 外周血

Designations: NC-37

NC-37細(xì)胞Depositors: J Wolff

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Disease: Burkitt's lymphoma

Cell Type: B lymphoblast;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NC-37細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Maywood New Jersey, United States

Isolation date: February, 1967

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 13,14

D16S539: 8,11

D5S818: 10,13

D7S820: 10

THO1: 6,7

TPOX: 8,13

vWA: 16

Cytogenetic Analysis: modal number = 48; range = 40 to 51

Isoenzymes: G6PD, B

Gender: male

Comments: NC-37細(xì)胞The NC-37 line has been utilized as a target cell for EBV superinfection studies and has proven useful in chemical induction studies of the latent EBV genome and in the propagation of various (retroviruses) oncornaviruses. The consistency of the line's characteristics has made it a useful tool in a wide variety of biochemical, immunological, virological and cell biological studies.

Each cell contains an average of 60 EBV genome copies. The cells are EBNA positive (EBNA+) and surface immunoglobulin negative (sIg-).

Although deposited with the ATCC as a lymphoblastoid cell line established from a 34 year old male caucasian, DNA fingerprinting has shown this line to be a derivative of Raji (see ATCC CCL-86), a Burkitt's lymphoma cell line.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Maintain cultures at 5 X 10(5) viable cells/ml. A maximum density of upto 2 X 10(6) is possible.

Medium Renewal: Add fresh medium (20% to 30% by volume) every 3 to 4 days

Preservation: Complete growth medium supplemented with 5% (v/v) DMSO.

Related Products: derivative:ATCC CCL-86

References: 22512: Durr FE, et al. Studies on the infectivity and cytopathology of Epstein-Barr virus in human lymphoblastoid cells. Int. J. Cancer 6: 436-449, 1970. PubMed: 4321017

26048: Mayyasi SA, et al. NC-37細(xì)胞The coating reaction of the herpes-type virus isolated from malignant tissues with an antibody present in sera. Cancer Res. 27: 2020-2024, 1967. PubMed: 6073499