NCI-H1238細胞

年限： stage E

運輸方式： 凍存運輸

是否是腫瘤細胞： 1

物種來源： 人

器官來源： 肺

數(shù)量： 大量

生長狀態(tài)： 懸浮，少量貼壁

ATCC Number： CRL-5859?

相關(guān)**： 小細胞肺癌

Designations: NCI-H1238 [H1238]

NCI-H1238細胞Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension; some adherent cells

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage E

Disease: carcinoma; small cell lung cancer

Derived from metastatic site: bone marrow

Permits/Forms: NCI-H1238細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 8,14

D16S539: 10

D5S818: 13

D7S820: 11

THO1: 9,9.3

TPOX: 8

vWA: 18,19

Age: 64 years

Gender: male

Ethnicity:NCI-H1238細胞 White

Comments: The line was established in November 1985.

The patient was a smoker.

130 pack years.

Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium

0.005 mg/ml Insulin

0.01 mg/ml Transferrin

30nM Sodium selenite (final conc.)

10 nM Hydrocortisone (final conc.)

10 nM beta-estradiol (final conc.)

extra 2mM L-glutamine (for final conc. of 4.5 mM)

NCI-H1238細胞5% fetal bovine serum (final conc.)

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Cultures can be maintained by addition of fresh medium.

Cells grow as floating aggregates of round cell clusters.

Some cells will attach.

Preservation: Culture medium with an additional 45% fetal bovine serum, 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.