NCI-H1930細胞

數(shù)量： 大量

ATCC Number： CRL-5906?

運輸方式： 凍存運輸

相關(guān)**： 小細胞肺癌

年限： stage L

器官來源： 肺

生長狀態(tài)： 懸浮生長，多細胞聚集

是否是腫瘤細胞： 1

物種來源： 人

Designations: NCI-H1930 [H1930]

Depositors: AF Gazdar, JD Minna

NCI-H1930細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage L

Disease: carcinoma; classic small cell lung cancer

Derived from metastatic site: lymph node

Permits/Forms: NCI-H1930細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

Cytogenetic Analysis: del(p13-p21)

Age: 41 years

Gender: male

Ethnicity: White

Comments: NCI-H1930細胞The line was established in May 1988.

The tissue donor was a non-smoker.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Note: Cells grow as floating aggregates of round cell clusters. Clusters are alive while single cells have very low viability. Do not try to break up the clusters as this would decrease the viability. Centrifuge cell suspension every 2-3 days. After centrifugation, aspirate supernatant down to 1 ml above cell pellet .Resuspend cells by gentle swirling in 3-4 ml of fresh medium. Seed at a concentration of 2-2.5 X 10(4) viable cells/ml. Transfer to new culture flasks when necessary

Preservation: Freeze medium: 50% FBS + 40% Culture Media + 10% DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23570: . NCI-H1930細胞NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.