NCI-H2195細(xì)胞

ATCC Number： CRL-5931?

相關(guān)**： 小細(xì)胞肺癌

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： stage E

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 肺

Designations: NCI-H2195 [H2195]

Depositors: AF Gazdar, JD Minna

NCI-H2195細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage E

Disease: carcinoma; small cell lung cancer

Derived from metastatic site: bone marrow

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: NCI-H2195細(xì)胞The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

Isolation: Isolation date: June, 1989

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 11

D16S539: 10

D5S818: 12

D7S820: 10,11

THO1: 9.3

TPOX: 8,9

vWA: 18

Age: 67 years *****

Gender: male

Ethnicity: Caucasian

Comments: NCI-H2195細(xì)胞A lymphoblastoid line from the same patient is available as ATCC CRL-5956.

Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium

0.005 mg/ml Insulin

0.01 mg/ml Transferrin

30nM Sodium selenite (final conc.)

10 nM Hydrocortisone (final conc.)

10 nM beta-estradiol (final conc.)

extra 2mM L-glutamine (for final conc. of 4.5 mM)

5% fetal bovine serum (final conc.)

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove medium, rinse with 0.25% (w/v) trypsin - 0.53 mM EDTA solution

Add 1 to 2 ml additional trypsin solution and allow flasks to remain at room temperature (or incubate at 37C) until cells detach.

Resuspend pellet in fresh medium and dispense into a culture flask.

Subcultivation Ratio: A ratio of 1:2 to 1:3 is recommended

Medium Renewal: Twice weekly

Preservation: Freeze medium: Culture medium, 90%; DMSO, 5%; FBS ,5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

L-Glutamine Solution, 200 mM:ATCC 30-2214

normal (or near-normal) cell line established from the same patient:ATCC CRL-5956

NCI-H2195細(xì)胞0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.