NCI-H295細胞

年限： 48 years

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

細胞形態(tài)： 上皮樣

器官來源： 腎上腺

ATCC Number： CRL-10296?

相關**： 其他**

組織來源： cortex

是否是腫瘤細胞： 1

物種來源： 人

NCI-H295細胞Designations: NCI-H295 [H295]

Depositors: AF Gazdar

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, with some loosely adherent cells, suspension with some loosely attached cells

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: adrenal gland

Tissue: cortex

Disease: adrenocortical carcinoma

Cellular Products: steroid hormones

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H295細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 13

D16S539: 11

D5S818: 12

D7S820: 9,12

THO1: 9.3

TPOX: 8

vWA: 17,18

Cytogenetic Analysis: modal number = 62; range = 55 to 64; hypertriploid. Twenty-three marker chromosomes were common to most cells: der(2)t(2;18)(p25;p11), del(3)(q21), der(10)t(10;13)(p15;q11), der(7)t(7;7)(q36;p13), del(7)(q11),; der(8)t(7;8)(p13;p23), del(8)(p12), del(9)(p22), del(9)(p13), del(11)(p12), del(12)(p12), t(13q18q) and 11 others. The del(9)(p13) was paired; normal N13 was absent. N15 had three copies and N22 had four copies.; NCI-H295細胞A total of 65 marker chromosomes were detected in 38 metaphases examined.; A minor subline that occurred in about 16% of the cells was characterized by the presence of del(M5)(7p11) replacing M5, by the single N17 (instead of paired N17) and by the presence of an additional M42.

Age: 48 years

Gender: female

Ethnicity: Black

Propagation: ATCC complete growth medium: HITES medium supplemented with 2% fetal bovine serum

HITES medium with 2% fetal bovine serum is formulated at the ATCC as follows:

Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006)

Insulin 0.005 mg/ml

Transferrin 0.01 mg/ml

Sodium selenite 30 nM

Hydrocortisone 10 nM

beta-estradiol 10 nM

HEPES 10 mM

L-glutamine 2 mM (in addition to that in the base medium)

fetal bovine serum 2%

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cells with some loosely attached cells. NCI-H295細胞Shake the flask to dislodge the cells and transfer to a new flask.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with an additional 8% fetal bovine serum and 5% DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 23082: Gazdar AF, et al. Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis. Cancer Res. 50: 5488-5496, 1990. PubMed: 2386954