NCI-H64細胞

ATCC Number： CRL-5976?

相關(guān)**： 小細胞肺癌

數(shù)量： 大量

運輸方式： 凍存運輸

是否是腫瘤細胞： 1

物種來源： 人

生長狀態(tài)： 漂浮生長

年限： stage E

器官來源： 肺

Designations: NCI-H64 [H64]

Depositors: AF Gazdar, JD Minna

NCI-H64細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: floating aggregates of round cell clusters

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage E

Disease: carcinoma; small cell lung cancer

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: NCI-H64細胞The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

Applications: The line was established in May 1977.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 10

D16S539: 9,12

D5S818: 11

D7S820: 10,12

THO1: 6

TPOX: 8

vWA: 17,18

Cytogenetic Analysis: 3p is normal

Age: 48 years

Gender: female

Ethnicity: Caucasian

Comments: NCI-H64細胞The line was established in May 1977.

The patient received prior chemotherapy and radiation therapy.

The patient was a smoker.

30 pack years.

Propagation: ATCC complete growth medium: HITES medium supplemented with 20% fetal bovine serum

HITES medium with 5% fetal bovine serum is formulated at the ATCC as follows:

Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006)

Insulin 0.005 mg/ml

Transferrin 0.01 mg/ml

Sodium selenite 30 nM

Hydrocortisone 10 nM

beta-estradiol 10 nM

HEPES 10 mM

L-glutamine 2 mM (in addition to that in the base medium)

fetal bovine serum 20%

Atmosphere: air, 95%; NCI-H64細胞carbon dioxide (CO2), 5%

Temperature: 37.0°C

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.