Hs 939.T細胞

生長狀態(tài)： 貼壁生長

年限： 24 years

細胞形態(tài)： 上皮樣

數(shù)量： 大量

運輸方式： 瓶裝運輸

ATCC Number： CRL-7690?

相關**： 黑色素瘤

是否是腫瘤細胞： 1

物種來源： 人

Designations: Hs 939.T

Biosafety Level: 1

Hs 939.T細胞Shipped: flask

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Disease: melanoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Cytogenetic Analysis: modal number = 46; range = 41 to 48

Age: 24 years

Gender: female

Ethnicity: Caucasian

Comments: Hs 939.T細胞Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Note: This item is distributed only within the 50 United States. It is not available for international distribution.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Hs 939.T細胞Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:Hs 939.T細胞ATCC 30-2020