Ca Ski細(xì)胞

細(xì)胞形態(tài)： 上皮樣

年限： 40 years *****

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 宮頸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-1550?

相關(guān)**： 上皮細(xì)胞癌

Designations: Ca Ski

Depositors: RA Pattillo

Ca Ski細(xì)胞Biosafety Level: 2 [Cells contain human papilloma virus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: cervix

Disease: epidermoid carcinoma

Derived from metastatic site: small intestine

Cellular Products: beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: Ca Ski細(xì)胞The line was established from cells from a metastasis in the small bowel mesentery.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 8,12

D16S539: 11,12

D5S818: 13

D7S820: 8,11

THO1: 7

TPOX: 8

vWA: 17

Isoenzymes: G6PD, B

Age: 40 years *****

Gender: female

Ethnicity: Caucasian

Comments: The line was established from cells from a metastasis in the small bowel mesentery.

The cells are reported to contain an integrated human papillomavirus type 16 genome (HPV-16, about 600 copies per cell) as well as sequences related to HPV-18.

Propagation: Ca Ski細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: 1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

also available as Certified Reference Material, ATCC CRM-CRL-1550

References: 1073: Pattillo RA, et al. Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line. Science 196: 1456-1458, 1977. Ca Ski細(xì)胞PubMed: 867042

22565: Baker CC, et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. PubMed: 3029430

22995: Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105