HT細(xì)胞

年限： 70 years

數(shù)量： 大量

ATCC Number： CRL-2260?

相關(guān)**： 其他**

組織來源： ascites

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： kappa

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞類型： B**細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來源： 人

HT細(xì)胞Designations: HT

Depositors: M Beckwith

Isotype: kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Tissue: ascites

Disease: diffuse mixed lymphoma

Cell Type: B lymphoblast;

Cellular Products: immunoglobulin; kappa light chain

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Isolation: HT細(xì)胞Isolation date: 1983

Antigen Expression: CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 13,14

D16S539: 11,13

D5S818: 11,13

D7S820: 8,10

THO1: 6,7

TPOX: 11

HT細(xì)胞vWA: 17,18

Age: 70 years

Gender: male

Ethnicity: Caucasian

Comments: HT is a human B cell lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.

HT cells express mRNA for both IgM and kappa and express low levels of kappa on the cell surface.

Exposure of HT cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.

HT cells have been reported to be Epstein-Barr virus genome negative.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10(5) viable cells/ml and maintain between 1 X 10(5) and 1 X 10(6).

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: HT細(xì)胞Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22555: Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723