A-204細胞

數(shù)量： 大量

運輸方式： 凍存運輸

ATCC Number： HTB-82?

相關**： 橫紋肌肉瘤

生長狀態(tài)： 貼壁生長

年限： 1 year

細胞形態(tài)： 上皮樣

組織來源： muscle

是否是腫瘤細胞： 1

物種來源： 人

Designations: A-204

A-204細胞Depositors: DJ Giard

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Tissue: muscle

Disease: rhabdomyosarcoma

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,13

D13S317: 11,12

D16S539: 11,12

A-204細胞D5S818: 12

D7S820: 8,10

THO1: 8,9.3

TPOX: 8,9

vWA: 15,17

Cytogenetic Analysis: diploidy and tetraploidy

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 1

PGM1, 1

PGM3, 1

Age: 1 year

Gender: female

Propagation: A-204細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Culture medium, 95%; DMSO, 5%

References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. A-204細胞One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758