DMS 79細(xì)胞

器官來源： 肺

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)，多細(xì)胞聚集

ATCC Number： CRL-2049?

相關(guān)**： 小細(xì)胞肺癌

是否是腫瘤細(xì)胞： 1

物種來源： 人

年限： 65 years

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

Designations: DMS 79

Depositors: OS Pettengill, G Sorenson

Biosafety Level: 1

DMS 79細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Disease: carcinoma; small cell lung cancer

Cellular Products: adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; corticotropin; beta endorphin; 17 beta estradiol; lipotropin; oxytocin - neurophysin (OT-NP); parathormone; somatostatin-like immunoreactivity (SRIF)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: Note: DMS 79細(xì)胞These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.

Receptors: epidermal growth factor (EGF)

Tumorigenic: Yes

Oncogene: c-myc +; N-myc +; c-raf-1 +; H-ras +; K-ras +; N-ras +; v-fes -; v-fms -

Antigen Expression: Leu 7; My23

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 11

D16S539: 12

D5S818: 10

D7S820: 9,11

THO1: 8

TPOX: 8

vWA: 18

Cytogenetic Analysis: DMS 79細(xì)胞Number of cells examined = 80; Modal Chromosome Number = 79 with a range of 65 to 84; Polyploidy Rate = 14.3%

Age: 65 years

Ethnicity: Caucasian

Comments: The line was established from cells in the pleural fluid of a patient with small cell carcinoma of the lung.

The patient had previously been treated with cytoxan, vincristine, methotrexate and radiation therapy.

The cell express HLA class I and class II antigens.

Derivative chromosomes observed at high frequency in 24 karyotyped metaphases included: der(2)t(2;?18)(q10;q10); del(3)?(pter-->p21::q10-->qter); del(6)(q13); der(10); add(11)(p15); der(14)t(14;17)(q10;q10); add(15)(p11); add(15)(p11)+; and del(17)(?q24).

Two copies of each derivative chromosome was observed in each metaphase.

Less frequent derivative chromosomes included: del(12)?(q11q21)[18/24 cells] and add(13)(p11)[10/24 cells]. Four to eight additional marker chromosomes of unknown origin were generally detected in each cell.

Cytogenetics Comments: This is a hyper-triploid human cancer cell line with a modal chromosome number of 79.

A Y chromosome was verified by QM staining and C-banding. Normal chromosomes 10, 15 and 17 were absent.

The composite karyotype consists of 65 to 84, XY, +1, -2, der(2)t(2;?18)(q10;q10)(x2); -3, del(3)?(pter-->p21::q10-->qter)x2, -4, -5, +6, del(6)(?q15)x2,+7,+8,-9,-10,-10,-10, der(10)x2, -11, add(11)(p15)x2, -12, del(12)?(q11q21)[18],

-13, +14, der(14)t(14;17)(q10;q10)x2, -15, -15,- 15, der(15)x2, -16, -17, -17, 17, del(17)x2, -18, +19, -20, +21, -22[cp24]

Propagation: DMS 79細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Cell counts are approximate since the cells grow in aggregates.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

References: 22250: Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

22793: Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631

23142: Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. PubMed: 6249655

24389: . . Lung Cancer 4: 155-161, 1988.