JAR細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： HTB-144?

器官來(lái)源： 胎盤

相關(guān)**： 其他**

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

年限： fetus

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

Designations: JAR

Depositors: RA Pattillo

Biosafety Level: 1

JAR細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: placenta

Disease: choriocarcinoma

Cellular Products: estrogen; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); hCG production averages 22.5 ng/ml after reculturing

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DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 7,10

D13S317: 11

D16S539: 9,10

D5S818: 10,11

D7S820: 10,11

THO1: 6,7

TPOX: 8,11

vWA: 16,18

Cytogenetic Analysis: JAR細(xì)胞This is probably a pseudotriploid human cell line with the modal chromosome number of 68, occurring in 24% of cells, but cells with both 69 (22%) and 70 (18%) chromosome counts also occurred frequently. Cells with higher ploidies occurred at 7.0%.

Karyotypes were extremely complex. Consistently there were 20 to 25 marker chromosomes (>30% of total chromosome content) per cell. Most marker chromosomes had complex structural rearrangements, and the origin of chromosome segments of these markers often defied identification. Among the frequently found markers were 8p+, 11p, a large metacentric [?t(3qter--3q12::?--C--?::3q12--3qter)] der(?13)T(1;?13) (p13;?q14), and many others. There was only one normal X chromosome. Normal N3 and N13 were not found.

Isoenzymes: AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 1

PGM1, 1-2

PGM3, 1-2

Age: fetus

Gender: male

Ethnicity: Caucasian

Propagation: JAR細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: JAR細(xì)胞Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 2156: Pattillo RA, et al. The Jar cell line -- continuous human multihormone production and controls. In Vitro 6: 398-399, 1971.