166-ME SK細(xì)胞
年限： 20 years
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CRL-1533?
相關(guān)**： 其他**
細(xì)胞形態(tài)： 成纖維樣
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
器官來(lái)源： 皮膚
166-ME SK細(xì)胞Designations: 166-ME SK
Depositors: L Kopelovich
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: hereditary adenomatosis (Gardner's variant)
166-ME SK細(xì)胞Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 20 years
Gender: male
Ethnicity: Black
Comments: The tissue donor suffered from Gardner's variant form of hereditary adenomatosis.
The line is capable of 16 population doublings after thawing.
Propagation:166-ME SK細(xì)胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. 166-ME SK細(xì)胞Subculture every 10 days.
