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產(chǎn)品名稱: 腫瘤壞死因子α(TNFa)單克隆抗體
產(chǎn)品型號: TNFa
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
腫瘤壞死因子α(TNFa)單克隆抗體是針對TNFa的小鼠單克隆抗體。在**組織化學(xué)染色和**印跡實(shí)驗(yàn)中能識別TNFa。腫瘤壞死因子α(TNFa)單克隆抗體主要用于WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM等多種**學(xué)實(shí)驗(yàn)，一抗，二抗，標(biāo)簽抗體，內(nèi)參抗體、信號通路抗體、細(xì)胞凋亡抗體等現(xiàn)貨供應(yīng)，獲得高靈敏度和高特異性的一致性結(jié)果的不二之選，產(chǎn)品靈敏度高，可重現(xiàn)性好，相同的名稱，不同的物種，多種規(guī)格供應(yīng),售后完善。
腫瘤壞死因子α(TNFa)單克隆抗體
的詳細(xì)介紹
腫瘤壞死因子α(TNFa)單克隆抗體
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來源單抗制備
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宿主小鼠
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效價(克隆號)D4
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Ig亞型IgG2a Kappa
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純化方式蛋白A + 蛋白G親和純化
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腫瘤壞死因子α(TNFa)單克隆抗體標(biāo)記物無標(biāo)記物
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度500ug/ml
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且適物種-
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應(yīng)用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
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腫瘤壞死因子α(TNFa)單克隆抗體Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Monoclonal antibody preparation
Host: Mouse
Antibody isotype: IgG2a Kappa
Purification: Protein A/G Affinity Chromatography. Clone number: D4
Traits: Liquid
Concentration: 500μg/mL
UOM: 200μg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: Recombinant TNFa (Val77~Leu233) expressed in E.coli. Accession No.: RPA133Hu01
[ APPLICATIONS ]
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,
50% glycerol.腫瘤壞死因子α(TNFa)單克隆抗體 [ QUALITY CONTROL ]
Content: The quality control contains recombinant TNFa disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition.腫瘤壞死因子α(TNFa)單克隆抗體 [ IDENTIFICATION ]
A
Figure 1. Western Blot
A. Sample: Recombinant TNFa, Human
