來源多抗制備
宿主兔
效價-
Ig類型IgG
純化方式抗原特異性親和純化
Ⅳ型膠原α5(COL4a5)多克隆抗體標(biāo)記物無標(biāo)記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
Ⅳ型膠原α5(COL4a5)多克隆抗體Polyclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: COL4a5, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
Ⅳ型膠原α5(COL4a5)多克隆抗體[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant COL4a5 (Gly1461~Thr1685) with two N-terminal
Tags, His-tag and S-tag expressed in E.coli.
Accession No.: RPC141Hu01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against COL4a5. It has been
selected for its ability to recognize COL4a5 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant COL4a5 (Gly1461~Thr1685)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,
BPB 0.01%, NaN3 0.02%.
Ⅳ型膠原α5(COL4a5)多克隆抗體[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant COL4a5, Human
Used in DAB staining on fromalin fixed
paraffin- embedded kidney tissue
PAC141Hu01
Polyclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
Used in Western Blot, Sample: Porcine Brain Tissue
Primary Ab: 1μg/mL Rabbit Anti-Human COL4a5 Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SECab05)