來源多抗制備
宿主兔
效價-
Ig類型IgG
純化方式抗原特異性親和純化
70kDa熱休克蛋白1B(HSPA1B)多克隆抗體標記物無標記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
70kDa熱休克蛋白1B(HSPA1B)多克隆抗體Polyclonal Antibody to Heat Shock 70kDa Protein 1B (HSPA1B)
Organism Species: Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: HSPA1B, Bovine
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg Sample: Recombinant HSPA1B, Bovine
70kDa熱休克蛋白1B(HSPA1B)多克隆抗體[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant HSPA1B (Met1~Asp641) expressed in E.coli. USCN Accession No.: RPA301Bo01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSGSEF-MAKNTAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS
YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFRVINDGDK
PKVQVSYKGE TKAFYPEEISSMVLTKMKEI AEAYLGHPVT NAVITVPAYF NDSQRQATKD
AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK
ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA
SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS
TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS
LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT RDNNLLGRFE
LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ
EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW
LDANTLAEKD EFEHKRKELE QVCNPIISRL YQGAGGPGAG GFGAQGPKGG SGSGPTIEEV
D
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against HSPA1B. It has been
selected for its ability to recognize HSPA1B in immunohistochemical staining and
western blotting.
70kDa熱休克蛋白1B(HSPA1B)多克隆抗體[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant HSPA1B (Met1~Asp641)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.